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Products
One-Step Swab/Saliva Viral RNA and DNA Purification Kit
Components
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Shipping conditions: At ambient temperature
Handling And Storage: Store The Kit Components According To The Table Above On Arrival.
Components
BcMag™ L27 Beads
10x Lysis Buffer
DTT
Storage
4°C
4°C
-20°C
Cat. No. AR101 (50 Preps)
2.5 ml
0.6 ml
15.4 mg
Cat. No. AR102 (100 Preps)
5.0 ml
1.2 ml
30.8 mg
Shipping conditions: At ambient temperature
Handling and Storage: Store the kit components according to the table Above on arrival.
Introduction
The accurate and rapid diagnosis of viral diseases is critical for early therapeutic intervention and effective treatment. Nucleic acid detections are among the most sensitive and specific tools for viral diagnosis, while the high quality of the purified nucleic acids is crucial for successful detection. Due to the very low amount of virus present in the samples, the nucleic acid purification techniques must be a robust and reliable extraction system to deliver pure and high-quality nucleic acids for sensitive downstream applications. Currently available viral Nucleic acid purification kits present challenges such as low DNA yield, time-consuming, laborious, or contamination with PCR inhibitors. They require expensive laboratory settings and well-trained personnel. To overcome these hurdles, Bioclone offers a novel extraction-free nucleic acid purification technique for trace amounts of viral samples, a robust alternative for viral nucleic acid extraction that guarantees a stable and scalable high-throughput extraction workflow at a low cost from the sample.
BcMag™ One-Step Swab/Saliva Viral RNA-DNA Purification Kit uses novel negative selection chromatography magnetic beads to quickly capture impurities such as PCR inhibitors from cell lysate, leaving the DNA /RNA untouched. Unlike standard tedious bind-wash-elute protocol, this convenient single-step and the extraction-free procedure does not contain traces of organic solvents, chaotropic salts, or EDTA, reducing the risk of DNA/RNA loss and carryover of extraction buffers, and recovers almost 100% DNA/RNA. The purification kit provides a fast and simple method for DNA/RNA purification with only one tube, no liquid transfer, and no requirement for carrier RNA. Hundreds of trace samples such as saliva and nasal-pharyngeal swabs in saline solution, nasal swabs, buccal swabs & sputum/saliva swabs can be processed in less than 15 minutes without using expensive types of equipment. The purified nucleic acid is ready for downstream applications: PCR, RT-PCR, RT-qPCR, LAMP, RT-LAMP.
Workflow
1.
Add cell lysis buffer to the sample and mix well.
2.
Incubate at room temperature for 5 minutes.
3.
Add the magetic beads and mix by pipetting or vortexing to capture the impurity.
4.
Magnetic capture the beads and aspirate the supernatant containing the pure ready-to-use DNA/RNA
Features and Advantages
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10-Minute Protocol: Our kit streamlines the extraction process, enabling you to obtain high-quality viral RNA/DNA in just 10 minutes, saving you valuable time.
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High-Quality Nucleic Acid: Trust in the quality of your extracted nucleic acids. Our kit consistently delivers high-quality RNA/DNA, meeting the stringent requirements of your research or diagnostic needs.
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One-Step, One-Tube: Extract viral RNA/DNA directly from swab and saliva samples, minimizing DNA loss, contamination risk, and handling steps
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Effectively removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc.
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Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.
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High Throughput: Designed for high-throughput applications, this kit allows you to process hundreds of samples simultaneously, making it ideal for large-scale testing or research projects. Compatible with many different automated liquid handling systems.